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anti ptch1 antibody  (Novus Biologicals)


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    Novus Biologicals anti ptch1 antibody
    Anti Ptch1 Antibody, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ptch1 antibody/product/Novus Biologicals
    Average 92 stars, based on 6 article reviews
    anti ptch1 antibody - by Bioz Stars, 2026-03
    92/100 stars

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    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, <t>PTCH1</t> and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.
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    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, <t>PTCH1</t> and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.
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    Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.

    Journal: International Journal of Molecular Sciences

    Article Title: Effects of IGF-1 on Proliferation, Angiogenesis, Tumor Stem Cell Populations and Activation of AKT and Hedgehog Pathways in Oral Squamous Cell Carcinoma

    doi: 10.3390/ijms21186487

    Figure Lengend Snippet: Regulation of AKT and HH pathways in SCC-4 by stimulation with IGF-1 or fibroblast-conditioned media. SCC-4 cells were stimulated for 24 h with 10% FBS, 100 ng/mL rIGF-1 or 50% conditioned medium from control fibroblasts (MF1) or cells overexpressing IGF-1 (MF1-IGF-1). Western blot analysis was performed to detect ( A ) total AKT and phospho-Akt (S473), with band intensity measured and plotted using ImageJ; ( B ) GLI1, IHH, PTCH1 and SMO band intensities were measured and plotted using ImageJ; ( C ) GLI1 mRNA expression was evaluated by RT-qPCR 6 h after stimulating cells with rIGF-1 or conditioned media. Data are presented as means ± SDs, bars represent comparisons between respective groups and (*) denotes statistical significance after applying the one-way ANOVA and Dunnett’s post-test, p < 0.05. CM: conditioned medium. ( D ) IHH immunostaining in MF1 and MF1-IGF-1 fibroblasts. The presence of the IHH ligand is shown in red, while nuclei were stained with DAPI (blue). Bars = 50 μm.

    Article Snippet: The following primary antibodies were used: GLI1 (1:500; Novus #NB800, Centennial, CO, USA), PTCH1 (1:500; Novus #NB200-118 Centennial, CO, USA), SHH (1:500; Novus #NBP2-22126, Centennial, CO, USA), IHH (1:500; Abcam #EP1192, Branford, CT, USA), pan-cytokeratin (1:500; MyBiosource IML-91, San Diego, CA, USA), Nanog (1:200; Santa Cruz SC1732, Dallas, TX, USA) and SOX2 (1:200; Millipore AB5731, Darmstadt, Alemanha).

    Techniques: Control, Western Blot, Expressing, Quantitative RT-PCR, Immunostaining, Staining